More than 130 suppliers
ISO certified
In-house tech support
we Connect you
Bio-Connect
ProductsBack/PUMAalpha (B-6) Alexa Fluor(r) 546

PUMAalpha (B-6) Alexa Fluor(r) 546

SC-377015AF546
Santa Cruz Biotechnology
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetBBC3
Price on request
200 ug
Large volume orders?
Order with a bulk request
More than 130 suppliers
ISO certified
In-house tech support

Overview

  • Supplier
    Santa Cruz Biotechnology
  • Product Name
    PUMAalpha (B-6) Alexa Fluor(r) 546
  • Delivery Days Customer
    5
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Conjugate
    AlexaFluor 546
  • Gene ID27113
  • Target name
    BBC3
  • Target description
    BCL2 binding component 3
  • Target synonyms
    JFY-1, JFY1, PUMA, bcl-2-binding component 3, p53 up-regulated modulator of apoptosis, p53-upregulated modulator of apoptosis
  • Host
    Mouse
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    12352203

FAQ

Go to our frequently asked questions

Related products

Product group Antibodies
RayBiotech
Anti-BBC3 Antibody144-64809
ApplicationsImmunoFluorescence, Western Blot, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetBBC3
  • SizePrice
Formalin-fixed and paraffin embedded rat brain labeled with Rabbit Anti BBC3/PUMA Polyclonal Antibody, Unconjugated (bs-1573R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Product group Antibodies
References
Bioss
BBC3/PUMA Polyclonal AntibodyBS-1573R
ApplicationsFlow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
ReactivityBovine, Canine, Human, Mouse, Porcine, Rat
TargetBBC3
  • SizePrice
Product group Antibodies
Everest Biotech
Goat anti-BBC3 AntibodyEB10389
ApplicationsWestern Blot, ELISA
ReactivityHuman
TargetBBC3
  • SizePrice
Product group Antibodies
Biomatik
BBC3 Polyclonal AntibodyCAC15800
ApplicationsWestern Blot, ELISA
ReactivityRat
TargetBBC3
  • SizePrice
Product group Antibodies
References
GeneTex
PUMA antibody [C2C3], C-termGTX109675
ApplicationsImmunoPrecipitation, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
ReactivityHuman
TargetBBC3
  • SizePrice
Anti-PUMA Antibody
Product group Antibodies
Antibodies.com
Anti-PUMA AntibodyA97952
ApplicationsWestern Blot, ELISA
ReactivityHuman, Mouse, Rat
  • SizePrice
Figure 1. Western blot analysis of PUMA/BBC3 using anti-PUMA/BBC3 antibody (A04899-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: human Hela whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PUMA/BBC3 antigen affinity purified polyclonal antibody (Catalog # A04899-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PUMA/BBC3 at approximately 18 kDa. The expected band size for PUMA/BBC3 is at 18 kDa.
Product group Antibodies
Boster Bio
Anti-PUMA/BBC3 Antibody Picoband(r)A04899-3-CARRIER-FREE
ApplicationsWestern Blot
ReactivityHuman
TargetBBC3
  • SizePrice
Western Blot analysis of 293 cells using PUMA Polyclonal Antibody
Product group Antibodies
Cusabio
BBC3 AntibodyCSB-PA003890
ApplicationsWestern Blot, ELISA
ReactivityHuman, Mouse, Rat
TargetBBC3
  • SizePrice
Product group Antibodies
LifeSpan BioSciences
BBC3 / PUMA AntibodyLS-C764933
ApplicationsImmunoHistoChemistry
ReactivityHuman
TargetBBC3
  • SizePrice
Product group Antibodies
Santa Cruz Biotechnology
PUMAalpha/beta (G-3)SC-374223
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ELISA
ReactivityHuman, Mouse, Rat
TargetBBC3
  • SizePrice