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ProductsBack/Purified Anti-Human CD11a Antibody [R7-1]

Purified Anti-Human CD11a Antibody [R7-1]

AGEL2389
Assay Genie
ApplicationsFlow Cytometry, Western Blot, ImmunoHistoChemistry
Product group Antibodies
TargetITGAL
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Overview

  • Supplier
    Assay Genie
  • Product Name
    Purified Anti-Human CD11a Antibody [R7-1]
  • Delivery Days Customer
    9
  • Applications
    Flow Cytometry, Western Blot, ImmunoHistoChemistry
  • Applications Supplier
    FCM, WB, IHC
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Gene ID3683
  • Target name
    ITGAL
  • Target description
    integrin subunit alpha L
  • Target synonyms
    antigen CD11A (p180), lymphocyte function-associated antigen 1, alpha polypeptide; CD11 antigen-like family member A; CD11A; integrin alpha-L; integrin gene promoter; integrin, alpha L (antigen CD11A (p180), lymphocyte function-associated antigen 1; alpha polypeptide); leukocyte adhesion glycoprotein LFA-1 alpha chain; leukocyte function-associated molecule 1 alpha chain; LFA-1; LFA-1 alpha; LFA1A; LFA-1A; lymphocyte function-associated antigen 1, alpha polypeptide
  • Host
    Mouse
  • Protein IDP20701
  • Protein Name
    Integrin alpha-L
  • Shelf life instruction
    12 months
  • Source
    Mouse
  • Reactivity Supplier
    Human
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of CD11a using anti-CD11a antibody (A04466-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. lane 1: JURKAT cell lysates, lane 2: CEM whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD11a antigen affinity purified polyclonal antibody (Catalog # A04466-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD11a at approximately 150KD. The expected band size for CD11a is at 129KD.
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