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ProductsBack/Pyrin / MEFV / MEF Antibody (aa5-39)

Pyrin / MEFV / MEF Antibody (aa5-39)

LS-C407879
LifeSpan BioSciences
ApplicationsWestern Blot
Product group Antibodies
ReactivityHuman, Rat
TargetMEFV
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    Pyrin / MEFV / MEF Antibody (aa5-39)
  • Delivery Days Customer
    23
  • Applications
    Western Blot
  • Applications Supplier
    WB (0.1 - 0.5 µg/ml)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID4210
  • Target name
    MEFV
  • Target description
    MEFV innate immunity regulator, pyrin
  • Target synonyms
    FMF, MEF, PAAND, TRIM20, pyrin, MEFV innate immuity regulator, pyrin, MEFV, pyrin innate immunity regulator, Mediterranean fever, marenostrin
  • Host
    Rabbit
  • Reactivity
    Human, Rat
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of Pyrin/MEFV using anti-Pyrin/MEFV antibody (A00764-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human U-87MG whole cell lysates, Lane 3: human Raji whole cell lysates, Lane 4: mouse spleen tissue lysates, Lane 5: mouse lung tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Pyrin/MEFV antigen affinity purified polyclonal antibody (Catalog # A00764-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Pyrin/MEFV at approximately 86 kDa. The expected band size for Pyrin/MEFV is at 86 kDa.
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