FACS analysis of Lovo cells using GTX60386 PDK1 antibody [4A11]. Green : PDK1 Purple : negative control
![ICC/IF analysis of HeLa cells using GTX60386 PDK1 antibody [4A11]. Green : PDK1 Blue: DRAQ5 fluorescent DNA dye Red: Actin filaments](https://www.genetex.com/upload/website/prouct_img/normal/GTX60386/GTX60386_20170912_ICCIF_w_23061123_662.webp "ICC/IF analysis of HeLa cells using GTX60386 PDK1 antibody [4A11]. Green : PDK1 Blue: DRAQ5 fluorescent DNA dye Red: Actin filaments")
![IHC-P analysis of breast cancer tissue (left) and brain tissue (right) using GTX60386 PDK1 antibody [4A11].](https://www.genetex.com/upload/website/prouct_img/normal/GTX60386/GTX60386_20170912_IHC-P_w_23061123_918.webp "IHC-P analysis of breast cancer tissue (left) and brain tissue (right) using GTX60386 PDK1 antibody [4A11].")
![WB analysis of NIH3T3 (1), HeLa (2), Jurkat (3), HepG2 (4), PC-12 (5), and Cos7 (6) cell lysate using GTX60386 PDK1 antibody [4A11].](https://www.genetex.com/upload/website/prouct_img/normal/GTX60386/GTX60386_20170912_WB_w_23061123_841.webp "WB analysis of NIH3T3 (1), HeLa (2), Jurkat (3), HepG2 (4), PC-12 (5), and Cos7 (6) cell lysate using GTX60386 PDK1 antibody [4A11].")
FACS analysis of Lovo cells using GTX60386 PDK1 antibody [4A11]. Green : PDK1 Purple : negative control
Pyruvate dehydrogenase kinase 1 / PDK1 antibody [4A11]
GTX60386
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Monkey, Mouse, Rat
TargetPDK1
Overview
- SupplierGeneTex
- Product NamePyruvate dehydrogenase kinase 1 / PDK1 antibody [4A11]
- Delivery Days Customer9
- Application Supplier NoteWB: 1/500 - 1/2000. ICC/IF: 1/200 - 1/1000. IHC-P: 1/200 - 1/1000. FACS: 1/200 - 1/400. ELISA: 1/10000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone ID4A11
- ConjugateUnconjugated
- Gene ID5163
- Target namePDK1
- Target descriptionpyruvate dehydrogenase kinase 1
- Target synonyms[Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 1, mitochondrial; mitochondrial pyruvate dehydrogenase, lipoamide, kinase isoenzyme 1; PDH kinase 1; pyruvate dehydrogenase (acetyl-transferring) kinase isozyme 1, mitochondrial; pyruvate dehydrogenase kinase, isoenzyme 1
- HostMouse
- IsotypeIgG1
- Protein IDQ15118
- Protein Name[Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 1, mitochondrial
- Scientific DescriptionPyruvate dehydrogenase (PDH) is a mitochondrial multienzyme complex that catalyzes the oxidative decarboxylation of pyruvate and is one of the major enzymes responsible for the regulation of homeostasis of carbohydrate fuels in mammals. The enzymatic activity is regulated by a phosphorylation/dephosphorylation cycle. Phosphorylation of PDH by a specific pyruvate dehydrogenase kinase (PDK) results in inactivation. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jun 2013]
- ReactivityHuman, Monkey, Mouse, Rat
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
References
- Determinants of resistance to VEGF-TKI and immune checkpoint inhibitors in metastatic renal cell carcinoma. Sharma R et al., 2021 Jun 7, J Exp Clin Cancer ResRead more