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ProductsBack/Pyruvate dehydrogenase kinase 1 / PDK1 antibody [4A11]
IHC-P analysis of breast cancer tissue (left) and brain tissue (right) using GTX60386 PDK1 antibody [4A11].
IHC-P analysis of breast cancer tissue (left) and brain tissue (right) using GTX60386 PDK1 antibody [4A11].
IHC-P analysis of breast cancer tissue (left) and brain tissue (right) using GTX60386 PDK1 antibody [4A11].

Pyruvate dehydrogenase kinase 1 / PDK1 antibody [4A11]

GTX60386
GeneTex
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Monkey, Mouse, Rat
TargetPDK1
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Overview

  • Supplier
    GeneTex
  • Product Name
    Pyruvate dehydrogenase kinase 1 / PDK1 antibody [4A11]
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1/500 - 1/2000. ICC/IF: 1/200 - 1/1000. IHC-P: 1/200 - 1/1000. FACS: 1/200 - 1/400. ELISA: 1/10000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    4A11
  • Conjugate
    Unconjugated
  • Gene ID5163
  • Target name
    PDK1
  • Target description
    pyruvate dehydrogenase kinase 1
  • Target synonyms
    [Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 1, mitochondrial, pyruvate dehydrogenase (acetyl-transferring) kinase isozyme 1, mitochondrial, PDH kinase 1, mitochondrial pyruvate dehydrogenase, lipoamide, kinase isoenzyme 1, pyruvate dehydrogenase kinase, isoenzyme 1
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDQ15118
  • Protein Name
    [Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 1, mitochondrial
  • Scientific Description
    Pyruvate dehydrogenase (PDH) is a mitochondrial multienzyme complex that catalyzes the oxidative decarboxylation of pyruvate and is one of the major enzymes responsible for the regulation of homeostasis of carbohydrate fuels in mammals. The enzymatic activity is regulated by a phosphorylation/dephosphorylation cycle. Phosphorylation of PDH by a specific pyruvate dehydrogenase kinase (PDK) results in inactivation. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jun 2013]
  • Reactivity
    Human, Monkey, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • Sharma R, Kadife E, Myers M, et al. Determinants of resistance to VEGF-TKI and immune checkpoint inhibitors in metastatic renal cell carcinoma. J Exp Clin Cancer Res. 2021,40(1):186. doi: 10.1186/s13046-021-01961-3
    Read this paper

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Figure 1. Western blot analysis of PDK1 using anti-PDK1 antibody (A01268-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HepG2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PDK1 antigen affinity purified polyclonal antibody (Catalog # A01268-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PDK1 at approximately 58 kDa. The expected band size for PDK1 is at 49 kDa.
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