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Lane 1: MCF-7; Lane 2: A431; Lane 3: A549; Lane 4: U-87MG cell lysate at 20 µg per lane, probed with bsm-51357M RAB13 (5C9) Monoclonal Antibody at 1:1000 dilution and 4℃ overnight incubation, followed by secondary antibody incubation for 60min at room temperature.
Lane 1: MCF-7; Lane 2: A431; Lane 3: A549; Lane 4: U-87MG cell lysate at 20 µg per lane, probed with bsm-51357M RAB13 (5C9) Monoclonal Antibody at 1:1000 dilution and 4℃ overnight incubation, followed by secondary antibody incubation for 60min at room temperature.
Lane 1: MCF-7; Lane 2: A431; Lane 3: A549; Lane 4: U-87MG cell lysate at 20 µg per lane, probed with bsm-51357M RAB13 (5C9) Monoclonal Antibody at 1:1000 dilution and 4℃ overnight incubation, followed by secondary antibody incubation for 60min at room temperature.

RAB13 (5C9) Monoclonal Antibody

BSM-51357M
Bioss Antibodies
ApplicationsWestern Blot
DownloadDatasheet
Product group Antibodies
TargetRAB13
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Overview

  • Supplier
    Bioss Antibodies
  • Product Name
    RAB13 (5C9) Monoclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    Western Blot
  • Applications Supplier
    WB(1:300-5000)
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    5C9
  • Concentration
    0.5 ug/ml
  • Conjugate
    Unconjugated
  • Gene ID5872
  • Target name
    RAB13
  • Target description
    RAB13, member RAS oncogene family
  • Target synonyms
    cell growth-inhibiting gene 4 protein; GIG4; growth-inhibiting gene 4 protein; RAS-associated protein RAB13; ras-related protein Rab-13
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDP51153
  • Protein Name
    Ras-related protein Rab-13
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of RAB13 using anti-RAB13 antibody (A04339). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human Caco-2 whole cell lysates, Lane 3: human MCF-7 whole cell lysates, Lane 4: human 293T whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RAB13 antigen affinity purified polyclonal antibody (Catalog # A04339) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RAB13 at approximately 23 kDa. The expected band size for RAB13 is at 23 kDa.
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