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ProductsBack/RAB27B Antibody - C-terminal region (OASF00069)

RAB27B Antibody - C-terminal region (OASF00069)

OASF00069
Aviva Systems Biology
ApplicationsImmunoFluorescence, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetRAB27B
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Overview

  • Supplier
    Aviva Systems Biology
  • Product Name
    RAB27B Antibody - C-terminal region (OASF00069)
  • Delivery Days Customer
    23
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    3 mg/ml
  • Conjugate
    Unconjugated
  • Format
    Liquid
  • Gene ID5874
  • Target name
    RAB27B
  • Target description
    RAB27B, member RAS oncogene family
  • Target synonyms
    C25KG, ras-related protein Rab-27B
  • Host
    Goat
  • Isotype
    IgG
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of RAB27B using anti-RAB27B antibody (A04890-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates, Lane 2: human PC-3 whole cell lysates, Lane 3: human U-87MG whole cell lysates, Lane 4: rat stomach tissue lysates, Lane 5: mouse stomach tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RAB27B antigen affinity purified polyclonal antibody (Catalog # A04890-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RAB27B at approximately 25 kDa. The expected band size for RAB27B is at 25 kDa.
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