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RAB5C Antibody

CSB-PA040136
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetRAB5C
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Overview

  • Supplier
    Cusabio
  • Product Name
    RAB5C Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID5878
  • Target name
    RAB5C
  • Target description
    RAB5C, member RAS oncogene family
  • Target synonyms
    L1880, RAB5CL, RAB5L, RABL, ras-related protein Rab-5C, RAB5C, member of RAS oncogene family
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP51148
  • Protein Name
    Ras-related protein Rab-5C
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of RAB5C/RABL using anti-RAB5C/RABL antibody (M05148-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human A549 whole cell lysates, Lane 4: human RT4 whole cell lysates, Lane 5: rat RH35 whole cell lysates, Lane 6: mouse HEPA1-6 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RAB5C/RABL antigen affinity purified monoclonal antibody (Catalog # M05148-1) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:1000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RAB5C/RABL at approximately 23 kDa. The expected band size for RAB5C/RABL is at 23 kDa.
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