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Rabbit anti-BubR1 Antibody, Affinity Purified

A300-995A
Bethyl Laboratories
ApplicationsImmunoPrecipitation, Western Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetBUB1B

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Overview

  • Supplier
    Bethyl Laboratories
  • Product Name
    Rabbit anti-BubR1 Antibody, Affinity Purified
  • Delivery Days Customer
    9
  • Applications
    ImmunoPrecipitation, Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    200 ug/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID701
  • Target name
    BUB1B
  • Target description
    BUB1 mitotic checkpoint serine/threonine kinase B
  • Target synonyms
    BUB1beta, BUBR1, Bub1A, MAD3L, MVA1, SSK1, hBUBR1, mitotic checkpoint serine/threonine-protein kinase BUB1 beta, BUB1B, mitotic checkpoint serine/threonine kinase, MAD3/BUB1-related protein kinase, budding uninhibited by benzimidazoles 1 homolog beta, mitotic checkpoint kinase MAD3L
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO60566
  • Protein Name
    Mitotic checkpoint serine/threonine-protein kinase BUB1 beta
  • Reactivity
    Human
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of BubR1/BUB1B using anti-BubR1/BUB1B antibody (A01564-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human Hek293 whole cell lysates, Lane 4: human K562 whole cell lysates, Lane 5: human HL-60 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BubR1/BUB1B antigen affinity purified polyclonal antibody (Catalog # A01564-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for BubR1/BUB1B at approximately 120-130KD. The expected band size for BubR1/BUB1B is at 120-130KD.
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