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Rabbit anti-CD1D Antibody, Affinity Purified

A304-422A
Bethyl Laboratories
ApplicationsWestern Blot
Product group Antibodies
TargetCD1D
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Overview

  • Supplier
    Bethyl Laboratories
  • Product Name
    Rabbit anti-CD1D Antibody, Affinity Purified
  • Delivery Days Customer
    9
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1000 ug/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID912
  • Target name
    CD1D
  • Target description
    CD1d molecule
  • Target synonyms
    antigen-presenting glycoprotein CD1d; CD1A; CD1D antigen, d polypeptide; differentiation antigen CD1-alpha-3; HMC class I antigen-like glycoprotein CD1D; R3; R3G1; T-cell surface glycoprotein CD1d; thymocyte antigen CD1D
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP15813
  • Protein Name
    Antigen-presenting glycoprotein CD1d
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of CD1D using anti-CD1D antibody (A00323). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat spleen tissue lysates, Lane 2: rat lung tissue lysates, Lane 3: rat heart tissue lysates, Lane 4: human THP-1 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD1D antigen affinity purified polyclonal antibody (Catalog # A00323) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD1D at approximately 55KD. The expected band size for CD1D is at 38KD.
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