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Rabbit anti-CD3E Antibody, Affinity Purified

A304-291A
Bethyl Laboratories
ApplicationsImmunoPrecipitation, Western Blot, ImmunoHistoChemistry
Product group Antibodies
TargetCD3E
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Overview

  • Supplier
    Bethyl Laboratories
  • Product Name
    Rabbit anti-CD3E Antibody, Affinity Purified
  • Delivery Days Customer
    9
  • Applications
    ImmunoPrecipitation, Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1000 ug/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID916
  • Target name
    CD3E
  • Target description
    CD3 epsilon subunit of T-cell receptor complex
  • Target synonyms
    CD3epsilon, IMD18, T3E, TCRE, T-cell surface glycoprotein CD3 epsilon chain, CD3-epsilon, CD3e antigen, epsilon polypeptide (TiT3 complex), CD3e molecule, epsilon (CD3-TCR complex), T-cell antigen receptor complex, epsilon subunit of T3, T-cell surface antigen T3/Leu-4 epsilon chain
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP07766
  • Protein Name
    T-cell surface glycoprotein CD3 epsilon chain
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of CD3E using anti-CD3E antibody (PB9093). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MOLT-4 whole cell lysates, Lane 2: human Jurkat whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD3E antigen affinity purified polyclonal antibody (Catalog # PB9093) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD3E at approximately 23 kDa. The expected band size for CD3E is at 23 kDa.
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