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Rabbit anti-Cyclin T1 Antibody, Affinity Purified

A303-496A
Bethyl Laboratories
ApplicationsImmunoPrecipitation, Western Blot, ImmunoHistoChemistry
Product group Antibodies
TargetCCNT1
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Overview

  • Supplier
    Bethyl Laboratories
  • Product Name
    Rabbit anti-Cyclin T1 Antibody, Affinity Purified
  • Delivery Days Customer
    9
  • Applications
    ImmunoPrecipitation, Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    200 ug/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID904
  • Target name
    CCNT1
  • Target description
    cyclin T1
  • Target synonyms
    CCNT, CYCT1, HIVE1, cyclin-T1, CDK9-associated C-type protein, MLLT10/CCNT1 fusion, cyclin C-related protein, human immunodeficiency virus type 1 (HIV-1) expression (elevated) 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO60563
  • Protein Name
    Cyclin-T1
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Cyclin T1 using anti-Cyclin T1 antibody (PB9990). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat kidney tissue lysates, Lane 2: mouse spleen tissue lysates, Lane 3: JURKAT whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Cyclin T1 antigen affinity purified polyclonal antibody (Catalog # PB9990) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Cyclin T1 at approximately 81 kDa. The expected band size for Cyclin T1 is at 81 kDa.
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