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ProductsBack/Rabbit anti-eIF2beta/EIF2S2 Antibody, Affinity Purified

Rabbit anti-eIF2beta/EIF2S2 Antibody, Affinity Purified

A301-743A
Bethyl Laboratories
ApplicationsImmunoPrecipitation, Western Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Primate, Rabbit
TargetEIF2S2

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Overview

  • Supplier
    Bethyl Laboratories
  • Product Name
    Rabbit anti-eIF2beta/EIF2S2 Antibody, Affinity Purified
  • Delivery Days Customer
    9
  • Applications
    ImmunoPrecipitation, Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    200 ug/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID8894
  • Target name
    EIF2S2
  • Target description
    eukaryotic translation initiation factor 2 subunit beta
  • Target synonyms
    EIF2, EIF2B, EIF2beta, PPP1R67, eIF-2-beta, eukaryotic translation initiation factor 2 subunit 2, eIF2-beta, eukaryotic translation initiation factor 2, subunit 2 beta, 38kDa, protein phosphatase 1, regulatory subunit 67
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP20042
  • Protein Name
    Eukaryotic translation initiation factor 2 subunit 2
  • Reactivity
    Human, Mouse, Primate, Rabbit
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of EIF2S2 using anti-EIF2S2 antibody (PA2157). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human Hela whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-EIF2S2 antigen affinity purified polyclonal antibody (Catalog # PA2157) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for EIF2S2 at approximately 38 kDa. The expected band size for EIF2S2 is at 38 kDa.
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