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ProductsBack/Rabbit anti-FKBP4/FKBP52 Antibody, Affinity Purified

Rabbit anti-FKBP4/FKBP52 Antibody, Affinity Purified

A301-426A
Bethyl Laboratories
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
TargetFKBP4
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Overview

  • Supplier
    Bethyl Laboratories
  • Product Name
    Rabbit anti-FKBP4/FKBP52 Antibody, Affinity Purified
  • Delivery Days Customer
    9
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    200 ug/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID2288
  • Target name
    FKBP4
  • Target description
    FKBP prolyl isomerase 4
  • Target synonyms
    FKBP51, FKBP52, FKBP59, HBI, Hsp56, PPIase, p52, peptidyl-prolyl cis-trans isomerase FKBP4, FK506 binding protein 4, 59kDa, HSP binding immunophilin, T-cell FK506-binding protein, 59kD, peptidylprolyl cis-trans isomerase, rotamase
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ02790
  • Protein Name
    Peptidyl-prolyl cis-trans isomerase FKBP4
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of FKBP52/FKBP4 using anti-FKBP52/FKBP4 antibody (A02165-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human PC-3 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human Jurkat whole cell lysates, Lane 4: human MCF-7 whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: rat testis tissue lysates, Lane 7: mouse brain tissue lysates, Lane 8: mouse testis tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FKBP52/FKBP4 antigen affinity purified polyclonal antibody (Catalog # A02165-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FKBP52/FKBP4 at approximately 56-59 kDa. The expected band size for FKBP52/FKBP4 is at 52 kDa.
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