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Rabbit anti-Ku70 Antibody, Affinity Purified

A302-623A
Bethyl Laboratories
ApplicationsWestern Blot
Product group Antibodies
TargetXRCC6
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Overview

  • Supplier
    Bethyl Laboratories
  • Product Name
    Rabbit anti-Ku70 Antibody, Affinity Purified
  • Delivery Days Customer
    9
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    200 ug/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID2547
  • Target name
    XRCC6
  • Target description
    X-ray repair cross complementing 6
  • Target synonyms
    5'-deoxyribose-5-phosphate lyase Ku70; 5'-dRP lyase Ku70; 70 kDa subunit of Ku antigen; ATP-dependent DNA helicase 2 subunit 1; ATP-dependent DNA helicase II, 70 kDa subunit; CTC box binding factor 75 kDa subunit; CTC75; CTCBF; DNA repair protein XRCC6; G22P1; Ku autoantigen p70 subunit; Ku autoantigen, 70kDa; KU70; lupus Ku autoantigen protein p70; ML8; thyroid autoantigen 70kD (Ku antigen); thyroid autoantigen 70kDa (Ku antigen); thyroid-lupus autoantigen p70; TLAA; X-ray repair complementing defective repair in Chinese hamster cells 6; X-ray repair cross-complementing protein 6
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP12956
  • Protein Name
    X-ray repair cross-complementing protein 6
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Ku70/XRCC6 using anti-Ku70/XRCC6 antibody (PB9520). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human LNCAP whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human 293T whole cell lysates, Lane 4: human HepG2 whole cell lysates, Lane 5: human Jurkat whole cell lysates, Lane 6: human K562 whole cell lysates, Lane 7: human A549 whole cell lysates, Lane 8: human A431 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Ku70/XRCC6 antigen affinity purified polyclonal antibody (Catalog # PB9520) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Ku70/XRCC6 at approximately 70 kDa. The expected band size for Ku70/XRCC6 is at 70 kDa.
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