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Rabbit anti-Mre11 Antibody, Affinity Purified

A303-998A
Bethyl Laboratories
ApplicationsFlow Cytometry, ImmunoPrecipitation, Western Blot, ImmunoHistoChemistry
Product group Antibodies
TargetMRE11
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Overview

  • Supplier
    Bethyl Laboratories
  • Product Name
    Rabbit anti-Mre11 Antibody, Affinity Purified
  • Delivery Days Customer
    9
  • Applications
    Flow Cytometry, ImmunoPrecipitation, Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1000 ug/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID4361
  • Target name
    MRE11
  • Target description
    MRE11 double strand break repair nuclease
  • Target synonyms
    ATLD, HNGS1, MRE11A, MRE11B, double-strand break repair protein MRE11, AT-like disease, DNA recombination and repair protein, MRE11 double strand break repair nuclease A, MRE11 homolog 1, MRE11 homolog A, double strand break repair nuclease, MRE11 homolog, double strand break repair nuclease A, MRE11 meiotic recombination 11 homolog A, MRE11 meiotic recombination 11-like protein A, double-strand break repair protein MRE11A, endo/exonuclease Mre11, meiotic recombination 11 homolog 1, meiotic recombination 11 homolog A
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP49959
  • Protein Name
    Double-strand break repair protein MRE11
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of MRE11 using anti-MRE11 antibody (A00731-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: human COLO-320 whole cell lysates, Lane 4: human U-87MG whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: rat liver tissue lysates, Lane 7: mouse brain tissue lysates, Lane 8: mouse liver tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MRE11 antigen affinity purified polyclonal antibody (Catalog # A00731-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MRE11 at approximately 81KD. The expected band size for MRE11 is at 81KD.
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