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Rabbit anti-NFATc1 Antibody, Affinity Purified

A303-508A
Bethyl Laboratories
ApplicationsImmunoPrecipitation, Western Blot
Product group Antibodies
TargetNFATC1
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Overview

  • Supplier
    Bethyl Laboratories
  • Product Name
    Rabbit anti-NFATc1 Antibody, Affinity Purified
  • Delivery Days Customer
    9
  • Applications
    ImmunoPrecipitation, Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1000 ug/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID4772
  • Target name
    NFATC1
  • Target description
    nuclear factor of activated T cells 1
  • Target synonyms
    NF-ATC, NF-ATc1.2, NFAT2, NFATc, nuclear factor of activated T-cells, cytoplasmic 1, NFAT transcription complex cytosolic component, nuclear factor of activated T-cells 'c', nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO95644
  • Protein Name
    Nuclear factor of activated T-cells, cytoplasmic 1
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of NFAT2 using anti-NFAT2 antibody (A00340-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: mouse thymus tissue lysates, Lane 2: human 22RV1 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NFAT2 antigen affinity purified polyclonal antibody (Catalog # A00340-1) at 0.5 ug/mL overnight at 4 then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NFAT2 at approximately 101KD. The expected band size for NFAT2 is at 101KD.
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