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Rabbit anti-PAK1 Antibody, Affinity Purified

A301-259A
Bethyl Laboratories
ApplicationsImmunoPrecipitation, Western Blot
Product group Antibodies
TargetPAK1
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Overview

  • Supplier
    Bethyl Laboratories
  • Product Name
    Rabbit anti-PAK1 Antibody, Affinity Purified
  • Delivery Days Customer
    9
  • Applications
    ImmunoPrecipitation, Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    200 ug/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID5058
  • Target name
    PAK1
  • Target description
    p21 (RAC1) activated kinase 1
  • Target synonyms
    IDDMSSD, PAKalpha, alpha-PAK, p65-PAK, serine/threonine-protein kinase PAK 1, STE20 homolog, yeast, p21 protein (Cdc42/Rac)-activated kinase 1, p21/Cdc42/Rac1-activated kinase 1 (STE20 homolog, yeast), p21/Cdc42/Rac1-activated kinase 1 (yeast Ste20-related)
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ13153
  • Protein Name
    Serine/threonine-protein kinase PAK 1
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of PAK1 using anti-PAK1 antibody (A00454-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: rat PC-12 whole cell lysates, Lane 3: mouse brain tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PAK1 antigen affinity purified polyclonal antibody (Catalog # A00454-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PAK1 at approximately 66KD. The expected band size for PAK1 is at 66KD.
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