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Rabbit anti-PLC-gamma 1 Antibody, Affinity Purified

A302-940A
Bethyl Laboratories
ApplicationsImmunoPrecipitation, Western Blot
Product group Antibodies
TargetPLCG1
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Overview

  • Supplier
    Bethyl Laboratories
  • Product Name
    Rabbit anti-PLC-gamma 1 Antibody, Affinity Purified
  • Delivery Days Customer
    9
  • Applications
    ImmunoPrecipitation, Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1000 ug/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID5335
  • Target name
    PLCG1
  • Target description
    phospholipase C gamma 1
  • Target synonyms
    IDAA, NCKAP3, PLC-II, PLC1, PLC148, PLCgamma1, 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase gamma-1, 1-phosphatidyl-D-myo-inositol-4,5-bisphosphate, PLC-148, PLC-gamma-1, inositoltrisphosphohydrolase, monophosphatidylinositol phosphodiesterase, phosphatidylinositol phospholipase C, phosphoinositidase C, phosphoinositide phospholipase C, phosphoinositide phospholipase C-gamma-1, phospholipase C, gamma 1 (formerly subtype 148), phospholipase C-148, phospholipase C-II, triphosphoinositide phosphodiesterase
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP19174
  • Protein Name
    1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase gamma-1
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of PLCG1 using anti-PLCG1 antibody (M00677). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: human SH-SY5Y whole cell lysates, Lane 5: rat C6 whole cell lysates, Lane 6: rat RH35 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PLCG1 antigen affinity purified monoclonal antibody (Catalog # M00677) at 1:1000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:1000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PLCG1 at approximately 149 kDa. The expected band size for PLCG1 is at 149 kDa.
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