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ProductsBack/Rabbit anti-PSMA2 Antibody, Affinity Purified

Rabbit anti-PSMA2 Antibody, Affinity Purified

A303-816A
Bethyl Laboratories
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
TargetPSMA2
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Overview

  • Supplier
    Bethyl Laboratories
  • Product Name
    Rabbit anti-PSMA2 Antibody, Affinity Purified
  • Delivery Days Customer
    9
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1000 ug/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID5683
  • Target name
    PSMA2
  • Target description
    proteasome 20S subunit alpha 2
  • Target synonyms
    HC3, MU, PMSA2, PSC2, proteasome subunit alpha type-2, alpha-2, macropain subunit C3, multicatalytic endopeptidase complex subunit C3, proteasome (prosome, macropain) subunit, alpha type, 2, proteasome component C3, proteasome subunit HC3, proteasome subunit alpha 2
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP25787
  • Protein Name
    Proteasome subunit alpha type-2
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of PSMA2 using anti-PSMA2 antibody (PB10088). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat testis tissue lysates, Lane 2: MCF-7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PSMA2 antigen affinity purified polyclonal antibody (Catalog # PB10088) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PSMA2 at approximately 26 kDa. The expected band size for PSMA2 is at 26 kDa.
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