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Rabbit anti-PSMA5 Antibody, Affinity Purified

A302-749A
Bethyl Laboratories
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
TargetPSMA5
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Overview

  • Supplier
    Bethyl Laboratories
  • Product Name
    Rabbit anti-PSMA5 Antibody, Affinity Purified
  • Delivery Days Customer
    9
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1000 ug/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID5686
  • Target name
    PSMA5
  • Target description
    proteasome 20S subunit alpha 5
  • Target synonyms
    PSC5, ZETA, proteasome subunit alpha type-5, alpha-5, epididymis tissue sperm binding protein Li 11n, macropain subunit zeta, macropain zeta chain, multicatalytic endopeptidase complex zeta chain, proteasome (prosome, macropain) subunit, alpha type, 5, proteasome alpha 5 subunit, proteasome component 5, proteasome subunit alpha 5, proteasome subunit zeta, proteasome zeta chain
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP28066
  • Protein Name
    Proteasome subunit alpha type-5
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    12352203

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Western Blot Positive WB detected in: A549 whole cell lysate, HepG2 whole cell lysate, Rat liver tissue All lanes: PSMA5 antibody at 3ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 27, 21 kDa Observed band size: 27 kDa
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Figure 1. Western blot analysis of PSMA5 using anti-PSMA5 antibody (A07499-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human 293T whole cell lysates, Lane 4: monkey COS-7 whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: rat PC-12 whole cell lysates, Lane 7: mouse brain tissue lysates, Lane 8: mouse Neuro-2a whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PSMA5 antigen affinity purified polyclonal antibody (Catalog # A07499-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PSMA5 at approximately 26 kDa. The expected band size for PSMA5 is at 26 kDa.
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Lane 1: Mouse Thymus tissue lysates; Lane 2: Mouse NIH/3T3 cell lysates; Lane 3: Rat Liver Tissue lysates; Lane 4: Rat Thymus tissue lysates probed with PSMA5 Polyclonal Antibody,Unconjugated (bs-9354R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
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Immunohistochemical staining of human cerebral cortex shows strong cytoplasmic and nuclear positivity in neuronal cells.
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