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ProductsBack/Rabbit anti-PSMB7 Antibody, Affinity Purified

Rabbit anti-PSMB7 Antibody, Affinity Purified

A303-848A
Bethyl Laboratories
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
TargetPSMB7
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Overview

  • Supplier
    Bethyl Laboratories
  • Product Name
    Rabbit anti-PSMB7 Antibody, Affinity Purified
  • Delivery Days Customer
    9
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1000 ug/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID5695
  • Target name
    PSMB7
  • Target description
    proteasome 20S subunit beta 7
  • Target synonyms
    proteasome subunit beta type-7, beta-2, epididymis secretory sperm binding protein, macropain chain Z, multicatalytic endopeptidase complex chain Z, proteasome (prosome, macropain) subunit, beta type, 7, proteasome catalytic subunit 2, proteasome subunit Z, proteasome subunit alpha, proteasome subunit beta 7, proteasome subunit beta-2, proteasome subunit beta2, protein serine kinase c17
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ99436
  • Protein Name
    Proteasome subunit beta type-7
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Proteasome 20S Beta 7/PSMB7 using anti-Proteasome 20S Beta 7/PSMB7 antibody (A08095-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human PC-3 whole cell lysates, Lane 3: human MCF-7 whole cell lysates, Lane 4: human Caco-2 whole cell lysates, Lane 5: human 293T whole cell lysates, Lane 6; human HepG2 whole cell lysates, Lane 7: human HL-60 whole cell lysates, Lane 8: human A549 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Proteasome 20S Beta 7/PSMB7 antigen affinity purified polyclonal antibody (Catalog # A08095-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Proteasome 20S Beta 7/PSMB7 at approximately 26 kDa. The expected band size for Proteasome 20S Beta 7/PSMB7 is at 26 kDa.
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