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Rabbit anti-PSMC4 Antibody, Affinity Purified

A303-849A
Bethyl Laboratories
ApplicationsImmunoPrecipitation, Western Blot, ImmunoHistoChemistry
Product group Antibodies
TargetPSMC4
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Overview

  • Supplier
    Bethyl Laboratories
  • Product Name
    Rabbit anti-PSMC4 Antibody, Affinity Purified
  • Delivery Days Customer
    9
  • Applications
    ImmunoPrecipitation, Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1000 ug/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID5704
  • Target name
    PSMC4
  • Target description
    proteasome 26S subunit, ATPase 4
  • Target synonyms
    MIP224, RPT3, S6, TBP-7, TBP7, 26S proteasome regulatory subunit 6B, 26S protease regulatory subunit 6B, 26S proteasome AAA-ATPase subunit RPT3, MB67-interacting protein, Tat-binding protein 7, protease 26S subunit 6, proteasome (prosome, macropain) 26S subunit, ATPase, 4
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP43686
  • Protein Name
    26S proteasome regulatory subunit 6B
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of PSMC4 using anti-PSMC4 antibody (A06099-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human U251 whole cell lysates, Lane 3: human U251 whole cell lysates, Lane 4: human A549 whole cell lysates, Lane 5: rat liver tissue lysates, Lane 6: rat brain tissue lysates, Lane 7: mouse liver tissue lysates, Lane 8: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PSMC4 antigen affinity purified polyclonal antibody (Catalog # A06099-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PSMC4 at approximately 50 kDa. The expected band size for PSMC4 is at 43-50 kDa.
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