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ProductsBack/Rabbit anti-RNF31 Antibody, Affinity Purified

Rabbit anti-RNF31 Antibody, Affinity Purified

A303-560A
Bethyl Laboratories
ApplicationsImmunoPrecipitation, Western Blot
Product group Antibodies
TargetRNF31
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Overview

  • Supplier
    Bethyl Laboratories
  • Product Name
    Rabbit anti-RNF31 Antibody, Affinity Purified
  • Delivery Days Customer
    9
  • Applications
    ImmunoPrecipitation, Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1000 ug/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID55072
  • Target name
    RNF31
  • Target description
    ring finger protein 31
  • Target synonyms
    E3 ubiquitin-protein ligase RNF31; HOIL-1-interacting protein; HOIP; Paul; RING-type E3 ubiquitin transferase RNF31; ZIBRA; zinc in-between-RING-finger ubiquitin-associated domain protein
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ96EP0
  • Protein Name
    E3 ubiquitin-protein ligase RNF31
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of RNF31 using anti-RNF31 antibody (A04457-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human placenta tissues lysates Lane 2: human HepG2 whole cell lysates Lane 3: human PC-3 whole cell lysates Lane 4: human A549 whole cell lysates Lane 5: human U2OS whole cell lysates Lane 6: human Caco-2 whole cell lysates Lane 7: human Hela whole cell lysates After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RNF31 antigen affinity purified polyclonal antibody (Catalog # A04457-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RNF31 at approximately 120KD. The expected band size for RNF31 is at 120KD.
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