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Rabbit anti-Sin3A Antibody, Affinity Purified

A300-724A
Bethyl Laboratories
ApplicationsImmunoPrecipitation, Western Blot, ImmunoHistoChemistry
Product group Antibodies
TargetSIN3A
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Overview

  • Supplier
    Bethyl Laboratories
  • Product Name
    Rabbit anti-Sin3A Antibody, Affinity Purified
  • Delivery Days Customer
    9
  • Applications
    ImmunoPrecipitation, Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    200 ug/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID25942
  • Target name
    SIN3A
  • Target description
    SIN3 transcription regulator family member A
  • Target synonyms
    CHR15DELq24, DEL15Q24, WITKOS, paired amphipathic helix protein Sin3a, SIN3 homolog A, transcription regulator, histone deacetylase complex subunit Sin3a, transcriptional co-repressor Sin3A, transcriptional corepressor Sin3a, transcriptional regulator, SIN3A
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ96ST3
  • Protein Name
    Paired amphipathic helix protein Sin3a
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of mSin3A/SIN3A using anti-mSin3A/SIN3A antibody (A01203-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample unde r reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human 293T whole cell lysates, Lane 4: human K562 whole cell lysates, Lane 5: human MCF-7 whole cell lysates, Lane 6: rat PC-12 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-mSin3A/SIN3A antigen affinity purified polyclonal antibody (Catalog # A01203-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for mSin3A/SIN3A at approximately 150 kDa. The expected band size for mSin3A/SIN3A is at 145 kDa.
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