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Western Blot Positive WB detected in: K562 whole cell lysate All lanes: SIN3A antibody at 2ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 146 kDa Observed band size: 146 kDa
Western Blot Positive WB detected in: K562 whole cell lysate All lanes: SIN3A antibody at 2ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 146 kDa Observed band size: 146 kDa
Western Blot Positive WB detected in: K562 whole cell lysate All lanes: SIN3A antibody at 2ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 146 kDa Observed band size: 146 kDa

SIN3A Antibody

CSB-PA836303LA01HU
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetSIN3A
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Overview

  • Supplier
    Cusabio
  • Product Name
    SIN3A Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID25942
  • Target name
    SIN3A
  • Target description
    SIN3 transcription regulator family member A
  • Target synonyms
    CHR15DELq24, DEL15Q24, WITKOS, paired amphipathic helix protein Sin3a, SIN3 homolog A, transcription regulator, histone deacetylase complex subunit Sin3a, transcriptional co-repressor Sin3A, transcriptional corepressor Sin3a, transcriptional regulator, SIN3A
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ96ST3
  • Protein Name
    Paired amphipathic helix protein Sin3a
  • Scientific Description
    Acts as a transcriptional repressor. Corepressor for REST. Interacts with MXI1 to repress MYC responsive genes and antagonize MYC oncogenic activities. Also interacts with MXD1-MAX heterodimers to repress transcription by tethering SIN3A to DNA. Acts cooperatively with OGT to repress transcription in parallel with histone deacetylation. Involved in he control of the circadian rhythms. Required for the transcriptional repression of circadian target genes, such as PER1, mediated by the large PER complex through histone deacetylation. Cooperates with FOXK1 to regulate cell cycle progression probably by repressing cell cycle inhibitor genes expression (By similarity).
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

FAQ

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Figure 1. Western blot analysis of mSin3A/SIN3A using anti-mSin3A/SIN3A antibody (A01203-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample unde r reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human 293T whole cell lysates, Lane 4: human K562 whole cell lysates, Lane 5: human MCF-7 whole cell lysates, Lane 6: rat PC-12 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-mSin3A/SIN3A antigen affinity purified polyclonal antibody (Catalog # A01203-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for mSin3A/SIN3A at approximately 150 kDa. The expected band size for mSin3A/SIN3A is at 145 kDa.
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