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Rabbit anti-STARD10 Antibody, Affinity Purified

A305-657A
Bethyl Laboratories
ApplicationsImmunoPrecipitation
Product group Antibodies
TargetSTARD10
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Overview

  • Supplier
    Bethyl Laboratories
  • Product Name
    Rabbit anti-STARD10 Antibody, Affinity Purified
  • Delivery Days Customer
    9
  • Applications
    ImmunoPrecipitation
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1000 ug/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID10809
  • Target name
    STARD10
  • Target description
    StAR related lipid transfer domain containing 10
  • Target synonyms
    CGI-52, NY-CO-28, PCTP2, SDCCAG28, START domain-containing protein 10, PCTP-L, PCTP-like protein, START domain containing 10, StAR-related lipid transfer (START) domain containing 10, antigen NY-CO-28, serologically defined colon cancer antigen 28, stAR-related lipid transfer protein 10
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9Y365
  • Protein Name
    START domain-containing protein 10
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of PCTP-L/STARD10 using anti-PCTP-L/STARD10 antibody (A10577-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human RT4 whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: human T47D whole cell lysates, Lane 4: rat liver tissue lysates, Lane 5: mouse liver tissue lysates, Lane 6: mouse HEPA1-6 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PCTP-L/STARD10 antigen affinity purified polyclonal antibody (Catalog # A10577-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PCTP-L/STARD10 at approximately 37 kDa. The expected band size for PCTP-L/STARD10 is at 33 kDa.
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