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Rabbit anti-TEIF Antibody, Affinity Purified

A302-652A
Bethyl Laboratories
ApplicationsImmunoPrecipitation
Product group Antibodies
TargetSCYL1
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Overview

  • Supplier
    Bethyl Laboratories
  • Product Name
    Rabbit anti-TEIF Antibody, Affinity Purified
  • Delivery Days Customer
    9
  • Applications
    ImmunoPrecipitation
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1000 ug/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID57410
  • Target name
    SCYL1
  • Target description
    SCY1 like pseudokinase 1
  • Target synonyms
    GKLP, HT019, NKTL, NTKL, P105, SCAR21, TAPK, TEIF, TRAP, N-terminal kinase-like protein, SCY1-like protein 1, SCY1-like, kinase-like 1, coated vesicle-associated kinase of 90 kDa, likely ortholog of mouse N-terminal kinase-like protein, telomerase regulation-associated protein, telomerase transcriptional elements-interacting factor, teratoma-associated tyrosine kinase
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ96KG9
  • Protein Name
    N-terminal kinase-like protein
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of SCYL1 using anti-SCYL1 antibody (A04750-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: human T-47D whole cell lysates, Lane 4: human PC-3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SCYL1 antigen affinity purified polyclonal antibody (Catalog # A04750-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SCYL1 at approximately 115 kDa. The expected band size for SCYL1 is at 90 kDa.
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