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ProductsBack/Rabbit anti-ZC3H7A Antibody, Affinity Purified

Rabbit anti-ZC3H7A Antibody, Affinity Purified

A305-148A
Bethyl Laboratories
ApplicationsWestern Blot
Product group Antibodies
TargetZC3H7A
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Overview

  • Supplier
    Bethyl Laboratories
  • Product Name
    Rabbit anti-ZC3H7A Antibody, Affinity Purified
  • Delivery Days Customer
    9
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1000 ug/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID29066
  • Target name
    ZC3H7A
  • Target description
    zinc finger CCCH-type containing 7A
  • Target synonyms
    HSPC055; ZC3H7; ZC3HDC7; zinc finger CCCH domain-containing protein 7A; zinc finger CCCH-type domain containing 7; zinc-finger protein AY163807
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ8IWR0
  • Protein Name
    Zinc finger CCCH domain-containing protein 7A
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of ZC3H7A using anti-ZC3H7A antibody (A15075-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ZC3H7A antigen affinity purified polyclonal antibody (Catalog # A15075-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ZC3H7A at approximately 111 kDa. The expected band size for ZC3H7A is at 111 kDa.
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