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ProductsBack/RAC antibody [23A8]

RAC antibody [23A8]

GTX13048
GeneTex
ApplicationsImmunoPrecipitation, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen
Product group Antibodies
TargetRAC1
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Overview

  • Supplier
    GeneTex
  • Product Name
    RAC antibody [23A8]
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 0.5-2.0 microg/ml. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoPrecipitation, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    23A8
  • Conjugate
    Unconjugated
  • Gene ID5879
  • Target name
    RAC1
  • Target description
    Rac family small GTPase 1
  • Target synonyms
    MIG5, MRD48, Rac-1, TC-25, p21-Rac1, ras-related C3 botulinum toxin substrate 1, cell migration-inducing gene 5 protein, ras-like protein TC25, ras-related C3 botulinum toxin substrate 1 (rho family, small GTP binding protein Rac1)
  • Host
    Mouse
  • Isotype
    IgG2b
  • Protein IDP63000
  • Protein Name
    Ras-related C3 botulinum toxin substrate 1
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

Datasheet

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Figure 1. Western blot analysis of Rac1 using anti-Rac1 antibody (PB9431). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A431 whole cell lysates, Lane 2: human Hacat whole cell lysates, Lane 3: human MCF-7 whole cell lysates, Lane 4: human PC-3 whole cell lysates, Lane 5: rat small intestine tissue lysates, Lane 6: mouse small intestine tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Rac1 antigen affinity purified polyclonal antibody (Catalog # PB9431) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Rac1 at approximately 21 kDa. The expected band size for Rac1 is at 21 kDa.
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