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Rac1 antibody

GTX100761
GeneTex
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetRAC1
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Overview

  • Supplier
    GeneTex
  • Product Name
    Rac1 antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:3000. IHC-P: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.79 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID5879
  • Target name
    RAC1
  • Target description
    Rac family small GTPase 1
  • Target synonyms
    cell migration-inducing gene 5 protein; MIG5; MRD48; p21-Rac1; Rac-1; ras-like protein TC25; ras-related C3 botulinum toxin substrate 1; ras-related C3 botulinum toxin substrate 1 (rho family, small GTP binding protein Rac1); TC-25
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP63000
  • Protein Name
    Ras-related C3 botulinum toxin substrate 1
  • Scientific Description
    The protein encoded by this gene is a GTPase which belongs to the RAS superfamily of small GTP-binding proteins. Members of this superfamily appear to regulate a diverse array of cellular events, including the control of cell growth, cytoskeletal reorganization, and the activation of protein kinases. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • Disruption of the pentraxin 3/CD44 interaction as an efficient therapy for triple-negative breast cancers. Hsiao YW et al., 2022 Jan, Clin Transl Med
    Read more
  • Rho family GTPases, Rac and Cdc42, control the localization of neonatal dentate granule cells during brain development. Ito H et al., 2019 Jul, Hippocampus
    Read more
  • Spatial Tissue Proteomics Quantifies Inter- and Intratumor Heterogeneity in Hepatocellular Carcinoma (HCC). Buczak K et al., 2018 Apr, Mol Cell Proteomics
    Read more
  • Antipsychotic drugs induce cell cytoskeleton reorganization in glial and neuronal cells via Rho/Cdc42 signal pathway. Chen ML et al., 2016 Nov 3, Prog Neuropsychopharmacol Biol Psychiatry
    Read more
  • Astrocytic CCAAT/Enhancer-Binding Protein Delta Contributes to Glial Scar Formation and Impairs Functional Recovery After Spinal Cord Injury. Wang SM et al., 2016 Nov, Mol Neurobiol
    Read more
  • 14-3-3epsilon and aeta regulate neurogenesis and differentiation of neuronal progenitor cells in the developing brain. Toyo-oka K et al., 2014 Sep 3, J Neurosci
    Read more
  • Targeting of Rac GTPases blocks the spread of intact human breast cancer. Katz E et al., 2012 Jun, Oncotarget
    Read more

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Figure 1. Western blot analysis of Rac1 using anti-Rac1 antibody (PB9431). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A431 whole cell lysates, Lane 2: human Hacat whole cell lysates, Lane 3: human MCF-7 whole cell lysates, Lane 4: human PC-3 whole cell lysates, Lane 5: rat small intestine tissue lysates, Lane 6: mouse small intestine tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Rac1 antigen affinity purified polyclonal antibody (Catalog # PB9431) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Rac1 at approximately 21 kDa. The expected band size for Rac1 is at 21 kDa.
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