Rad21 antibody [HL3416] detects Rad21 protein by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Rad21 stained by Rad21 antibody [HL3416] (GTX641258) diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
![Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Rad21 antibody [HL3416] (GTX641258) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX641258/GTX641258_T-45586_20241220_WB_24122400_914.webp "Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Rad21 antibody [HL3416] (GTX641258) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.")
![Rad21 antibody [HL3416] detects Rad21 protein by immunohistochemical analysis. Sample: Paraffin-embedded human placenta. Rad21 stained by Rad21 antibody [HL3416] (GTX641258) diluted at 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](https://www.genetex.com/upload/website/prouct_img/normal/GTX641258/GTX641258_T-45586_20241227_IHC-P_25012200_434.webp "Rad21 antibody [HL3416] detects Rad21 protein by immunohistochemical analysis. Sample: Paraffin-embedded human placenta. Rad21 stained by Rad21 antibody [HL3416] (GTX641258) diluted at 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![Rad21 antibody [HL3416] detects Rad21 protein by immunohistochemical analysis. Sample: Paraffin-embedded mouse tissues. Rad21 stained by Rad21 antibody [HL3416] (GTX641258) diluted at 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min Corresponding RNA levels (RPKM) in the tissues are based on NCBI database.](https://www.genetex.com/upload/website/prouct_img/normal/GTX641258/GTX641258_T-45586_20250206_IHC-P_M_Multiple_RPKM_25022521_979.webp "Rad21 antibody [HL3416] detects Rad21 protein by immunohistochemical analysis. Sample: Paraffin-embedded mouse tissues. Rad21 stained by Rad21 antibody [HL3416] (GTX641258) diluted at 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min Corresponding RNA levels (RPKM) in the tissues are based on NCBI database.")
Rad21 antibody [HL3416] detects Rad21 protein by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Rad21 stained by Rad21 antibody [HL3416] (GTX641258) diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Rad21 antibody [HL3416]
GTX641258
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse
TargetRAD21
Overview
- SupplierGeneTex
- Product NameRad21 antibody [HL3416]
- Delivery Days Customer9
- Application Supplier NoteICC/IF: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDHL3416
- Concentration1 mg/ml
- ConjugateUnconjugated
- Gene ID5885
- Target nameRAD21
- Target descriptionRAD21 cohesin complex component
- Target synonymsCDLS4, HR21, HRAD21, MCD1, MGS, NXP1, SCC1, hHR21, double-strand-break repair protein rad21 homolog, NXP-1, RAD21 homolog, SCC1 homolog, kleisin, nuclear matrix protein 1, protein involved in DNA double-strand break repair, sister chromatid cohesion 1
- HostRabbit
- IsotypeIgG
- Protein IDO60216
- Protein NameDouble-strand-break repair protein rad21 homolog
- Scientific DescriptionThe protein encoded by this gene is highly similar to the gene product of Schizosaccharomyces pombe rad21, a gene involved in the repair of DNA double-strand breaks, as well as in chromatid cohesion during mitosis. This protein is a nuclear phospho-protein, which becomes hyperphosphorylated in cell cycle M phase. The highly regulated association of this protein with mitotic chromatin specifically at the centromere region suggests its role in sister chromatid cohesion in mitotic cells. [provided by RefSeq, Jul 2008]
- ReactivityHuman, Mouse
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
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