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Western blot All lanes: RAD23A antibody at 3microg/ml Lane 1: MCF-7 whole cell lysate Lane 2: Hela whole cell lysate Lane 3: Raji whole cell lysate Lane 4: Jurkat whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 40, 34 kDa Observed band size: 34 kDa
Western blot All lanes: RAD23A antibody at 3microg/ml Lane 1: MCF-7 whole cell lysate Lane 2: Hela whole cell lysate Lane 3: Raji whole cell lysate Lane 4: Jurkat whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 40, 34 kDa Observed band size: 34 kDa
Western blot All lanes: RAD23A antibody at 3microg/ml Lane 1: MCF-7 whole cell lysate Lane 2: Hela whole cell lysate Lane 3: Raji whole cell lysate Lane 4: Jurkat whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 40, 34 kDa Observed band size: 34 kDa

RAD23A Antibody

CSB-PA03774A0RB
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetRAD23A
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Overview

  • Supplier
    Cusabio
  • Product Name
    RAD23A Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID5886
  • Target name
    RAD23A
  • Target description
    RAD23 homolog A, nucleotide excision repair protein
  • Target synonyms
    HHR23A; HR23A; RAD23, yeast homolog, A; UV excision repair protein RAD23 homolog A
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP54725
  • Protein Name
    UV excision repair protein RAD23 homolog A
  • Scientific Description
    Multiubiquitin chain receptor involved in modulation of proteasomal degradation. Binds to Lys-48-linked polyubiquitin chains in a length-dependent manner and with a lower affinity to Lys-63-linked polyubiquitin chains. Proposed to be capable to bind simultaneously to the 26S proteasome and to polyubiquitinated substrates and to deliver ubiquitinated proteins to the proteasome. Involved in nucleotide excision repair and is thought to be functional equivalent for RAD23B in global genome nucleotide excision repair (GG-NER) by association with XPC. In vitro, the XPC:RAD23A dimer has NER activity. Can stabilize XPC. (Microbial infection) Involved in Vpr-dependent replication of HIV-1 in non-proliferating cells and primary macrophages. Required for the association of HIV-1 Vpr with the host proteasome.
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of HHR23A/RAD23A using anti-HHR23A/RAD23A antibody (A03243-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human MCF-7 whole cell lysates, Lane 4: human Jurkat whole cell lysates, Lane 5: rat testis tissue lysates, Lane 6: mouse testis tissue lysates, Lane 7: mouse lung tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HHR23A/RAD23A antigen affinity purified polyclonal antibody (Catalog # A03243-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HHR23A/RAD23A at approximately 60 kDa. The expected band size for HHR23A/RAD23A is at 60 kDa.
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