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RAD23A Polyclonal Antibody

CAC14049
Biomatik
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetRAD23A
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Overview

  • Supplier
    Biomatik
  • Product Name
    RAD23A Polyclonal Antibody
  • Delivery Days Customer
    12
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID5886
  • Target name
    RAD23A
  • Target description
    RAD23 homolog A, nucleotide excision repair protein
  • Target synonyms
    HHR23A; HR23A; RAD23, yeast homolog, A; UV excision repair protein RAD23 homolog A
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP54725
  • Protein Name
    UV excision repair protein RAD23 homolog A
  • Scientific Description
    The RAD23A Polyclonal Antibody (Species: Human) has been validated for the following applications: ELISA, WB, IHC.
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Western blot All lanes: RAD23A antibody at 3microg/ml Lane 1: MCF-7 whole cell lysate Lane 2: Hela whole cell lysate Lane 3: Raji whole cell lysate Lane 4: Jurkat whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 40, 34 kDa Observed band size: 34 kDa
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Figure 1. Western blot analysis of HHR23A/RAD23A using anti-HHR23A/RAD23A antibody (A03243-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human MCF-7 whole cell lysates, Lane 4: human Jurkat whole cell lysates, Lane 5: rat testis tissue lysates, Lane 6: mouse testis tissue lysates, Lane 7: mouse lung tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HHR23A/RAD23A antigen affinity purified polyclonal antibody (Catalog # A03243-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HHR23A/RAD23A at approximately 60 kDa. The expected band size for HHR23A/RAD23A is at 60 kDa.
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