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ProductsBack/Raf1 (phospho Ser338/Tyr340) antibody
WB analysis of HEK293 cells overexpressing c-Raf alone (Lane 1) or immunoprecipitates prepared from cells co-transfected with c-Raf, Ras (V12), and Rac1 (Lane 2-6) using GTX24768 Raf1 (phospho Ser338/Tyr340) antibody prior incubated with the non-phosphopeptide corresponding to the phosphopeptide immunogen (Lane 3), a generic phosphoserine-containing peptide (Lane 4), a generic phosphotyrosine-containing peptide (Lane 5), or the phosphopeptide immunogen (Lane 6). The data show that only the immunogen phosphopeptide blocks the signal, demonstrating the specificity of the antibody.
WB analysis of HEK293 cells overexpressing c-Raf alone (Lane 1) or immunoprecipitates prepared from cells co-transfected with c-Raf, Ras (V12), and Rac1 (Lane 2-6) using GTX24768 Raf1 (phospho Ser338/Tyr340) antibody prior incubated with the non-phosphopeptide corresponding to the phosphopeptide immunogen (Lane 3), a generic phosphoserine-containing peptide (Lane 4), a generic phosphotyrosine-containing peptide (Lane 5), or the phosphopeptide immunogen (Lane 6). The data show that only the immunogen phosphopeptide blocks the signal, demonstrating the specificity of the antibody.
WB analysis of HEK293 cells overexpressing c-Raf alone (Lane 1) or immunoprecipitates prepared from cells co-transfected with c-Raf, Ras (V12), and Rac1 (Lane 2-6) using GTX24768 Raf1 (phospho Ser338/Tyr340) antibody prior incubated with the non-phosphopeptide corresponding to the phosphopeptide immunogen (Lane 3), a generic phosphoserine-containing peptide (Lane 4), a generic phosphotyrosine-containing peptide (Lane 5), or the phosphopeptide immunogen (Lane 6). The data show that only the immunogen phosphopeptide blocks the signal, demonstrating the specificity of the antibody.

Raf1 (phospho Ser338/Tyr340) antibody

GTX24768
GeneTex
ApplicationsWestern Blot
Product group Antibodies
TargetRAF1
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Overview

  • Supplier
    GeneTex
  • Product Name
    Raf1 (phospho Ser338/Tyr340) antibody
  • Delivery Days Customer
    9
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID5894
  • Target name
    RAF1
  • Target description
    Raf-1 proto-oncogene, serine/threonine kinase
  • Target synonyms
    CMD1NN, CRAF, NS5, Raf-1, c-Raf, RAF proto-oncogene serine/threonine-protein kinase, C-Raf proto-oncogene, serine/threonine kinase, Oncogene RAF1, proto-oncogene c-RAF, raf proto-oncogene serine/threonine protein kinase, v-raf-1 murine leukemia viral oncogene homolog 1, v-raf-1 murine leukemia viral oncogene-like protein 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP04049
  • Protein Name
    RAF proto-oncogene serine/threonine-protein kinase
  • Scientific Description
    This gene is the cellular homolog of viral raf gene (v-raf). The encoded protein is a MAP kinase kinase kinase (MAP3K), which functions downstream of the Ras family of membrane associated GTPases to which it binds directly. Once activated, the cellular RAF1 protein can phosphorylate to activate the dual specificity protein kinases MEK1 and MEK2, which in turn phosphorylate to activate the serine/threonine specific protein kinases, ERK1 and ERK2. Activated ERKs are pleiotropic effectors of cell physiology and play an important role in the control of gene expression involved in the cell division cycle, apoptosis, cell differentiation and cell migration. Mutations in this gene are associated with Noonan syndrome 5 and LEOPARD syndrome 2. [provided by RefSeq, Jul 2008]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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