WB analysis of immunoprecipitates prepared HEK293 cells overexpressing wild-type c-Raf and stimulated with EGF (Lane 1-4) or c-Raf mutant S621A (Lane 5) using GTX24767 Raf1 (phospho Ser621) antibody.
WB analysis of immunoprecipitates prepared HEK293 cells overexpressing wild-type c-Raf and stimulated with EGF (Lane 1-4) or c-Raf mutant S621A (Lane 5) using GTX24767 Raf1 (phospho Ser621) antibody.
Raf1 (phospho Ser621) antibody - Orthogonal, Overexpression Validated
GTX24767
ApplicationsFlow Cytometry, ImmunoPrecipitation, Western Blot
Product group Antibodies
ReactivityHuman, Rat
TargetRAF1
Overview
- SupplierGeneTex
- Product NameRaf1 (phospho Ser621) antibody - Orthogonal, Overexpression Validated
- Delivery Days Customer9
- ApplicationsFlow Cytometry, ImmunoPrecipitation, Western Blot
- CertificationResearch Use Only
- ClonalityPolyclonal
- ConjugateUnconjugated
- Gene ID5894
- Target nameRAF1
- Target descriptionRaf-1 proto-oncogene, serine/threonine kinase
- Target synonymsCMD1NN; CRAF; c-Raf; C-Raf proto-oncogene, serine/threonine kinase; NS5; Oncogene RAF1; proto-oncogene c-RAF; raf proto-oncogene serine/threonine protein kinase; RAF proto-oncogene serine/threonine-protein kinase; Raf-1; v-raf-1 murine leukemia viral oncogene homolog 1; v-raf-1 murine leukemia viral oncogene-like protein 1
- HostRabbit
- IsotypeIgG
- Modification TypePhosphorylated
- Scientific DescriptionThis gene is the cellular homolog of viral raf gene (v-raf). The encoded protein is a MAP kinase kinase kinase (MAP3K), which functions downstream of the Ras family of membrane associated GTPases to which it binds directly. Once activated, the cellular RAF1 protein can phosphorylate to activate the dual specificity protein kinases MEK1 and MEK2, which in turn phosphorylate to activate the serine/threonine specific protein kinases, ERK1 and ERK2. Activated ERKs are pleiotropic effectors of cell physiology and play an important role in the control of gene expression involved in the cell division cycle, apoptosis, cell differentiation and cell migration. Mutations in this gene are associated with Noonan syndrome 5 and LEOPARD syndrome 2. [provided by RefSeq, Jul 2008]
- ReactivityHuman, Rat
- Storage Instruction2°C to 8°C,-20°C or -80°C
- UNSPSC12352203
References
- Platinum-Based Modification of Styrylbenzylsulfones as Multifunctional Antitumor Agents: Targeting the RAS/RAF Pathway, Enhancing Antitumor Activity, and Overcoming Multidrug Resistance. Liu Z et al., 2020 Jan 9, J Med ChemRead more