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ICC/IF analysis of U2OS cells using GTX03249 RALBP1 antibody [GT1337]. Blue : DAPI for nuclear staining Dilution : 1:100
ICC/IF analysis of U2OS cells using GTX03249 RALBP1 antibody [GT1337]. Blue : DAPI for nuclear staining Dilution : 1:100
ICC/IF analysis of U2OS cells using GTX03249 RALBP1 antibody [GT1337]. Blue : DAPI for nuclear staining Dilution : 1:100

RALBP1 antibody [GT1337]

GTX03249
GeneTex
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetRALBP1
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Overview

  • Supplier
    GeneTex
  • Product Name
    RALBP1 antibody [GT1337]
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500 - 1:2000. ICC/IF: 1:50 - 1:200. IHC-P: 1:50 - 1:200. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    GT1337
  • Concentration
    0.24 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID10928
  • Target name
    RALBP1
  • Target description
    ralA binding protein 1
  • Target synonyms
    76 kDa Ral-interacting protein; dinitrophenyl S-glutathione ATPase; DNP-SG ATPase; ralA-binding protein 1; ral-interacting protein 1; RIP1; RLIP1; RLIP76
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ15311
  • Protein Name
    RalA-binding protein 1
  • Scientific Description
    RALBP1 plays a role in receptor-mediated endocytosis and is a downstream effector of the small GTP-binding protein RAL (see RALA; MIM 179550). Small G proteins, such as RAL, have GDP-bound inactive and GTP-bound active forms, which shift from the inactive to the active state through the action of RALGDS (MIM 601619), which in turn is activated by RAS (see HRAS; MIM 190020) (summary by Feig, 2003 [PubMed 12888294]).[supplied by OMIM, Nov 2010]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of RALBP1 using anti-RALBP1 antibody (A01403-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human placenta tissue lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: human SGC-7901 whole cell lysates, Lane 5: human 22RV1 whole cell lysates, Lane 6: rat lung tissue lysates, Lane 7: mouse lung tissue lysates, Lane 8: mouse testis tissue lysates, Lane 9: mouse ovary tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RALBP1 antigen affinity purified polyclonal antibody (Catalog # A01403-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RALBP1 at approximately 95KD. The expected band size for RALBP1 is at 76KD.
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