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ELISA analysis of human RANTES recombinant proteint (0.2 - 0.4 ng/well) using GTX12766 RANTES antibody (Biotin). Working concentration : 0.25- 1.0 ug/ml
ELISA analysis of human RANTES recombinant proteint (0.2 - 0.4 ng/well) using GTX12766 RANTES antibody (Biotin). Working concentration : 0.25- 1.0 ug/ml
ELISA analysis of human RANTES recombinant proteint (0.2 - 0.4 ng/well) using GTX12766 RANTES antibody (Biotin). Working concentration : 0.25- 1.0 ug/ml

RANTES antibody (Biotin)

GTX12766
GeneTex
ApplicationsWestern Blot, ELISA
Product group Antibodies
TargetCCL5
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Overview

  • Supplier
    GeneTex
  • Product Name
    RANTES antibody (Biotin)
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 0.1 - 0.2 microg/mL. ELISA: 0.15 - 0.30 ng/mL. Sandwich ELISA: 0.25 - 1.0 ug/ml. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Biotin
  • Gene ID6352
  • Target name
    CCL5
  • Target description
    C-C motif chemokine ligand 5
  • Target synonyms
    beta-chemokine RANTES; C-C motif chemokine 5; chemokine (C-C motif) ligand 5; D17S136E; eoCP; eosinophil chemotactic cytokine; RANTES; regulated upon activation, normally T-expressed, and presumably secreted; SCYA5; SISd; SIS-delta; small inducible cytokine subfamily A (Cys-Cys), member 5; T-cell specific protein p288; TCP228
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP13501
  • Protein Name
    C-C motif chemokine 5
  • Scientific Description
    RANTES is a chemoattractant for blood monocytes, memory T helper cells and eosinophils. It causes the release of histamine from basophils and activates eosinophils. It binds to CCR1, CCR3, CCR4 and CCR5 and is one of the major HIV-suppressive factors produced by CD8+ T cells. Recombinant RANTES protein induces a dose-dependent inhibition of different strains of HIV-1, HIV-2, and simian immunodeficiency virus (SIV). It is T-cell and macrophage specific, induced by mitogens, and belongs to the intercrine beta (chemokine CC) family.
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of RANTES using anti-RANTES antibody (PB9573). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: HUT Whole Cell Lysate at 40ug. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RANTES antigen affinity purified polyclonal antibody (Catalog # PB9573) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RANTES at approximately 11 kDa. The expected band size for RANTES is at 11 kDa.
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