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Western Blot analysis of COLO205 cells using Rap1GAP Polyclonal Antibody
Western Blot analysis of COLO205 cells using Rap1GAP Polyclonal Antibody
Western Blot analysis of COLO205 cells using Rap1GAP Polyclonal Antibody

RAP1GAP Antibody

CSB-PA003941
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse
TargetRAP1GAP
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Overview

  • Supplier
    Cusabio
  • Product Name
    RAP1GAP Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID5909
  • Target name
    RAP1GAP
  • Target description
    RAP1 GTPase activating protein
  • Target synonyms
    RAP1GA1, RAP1GAP1, RAP1GAPII, RAPGAP, rap1 GTPase-activating protein 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP47736
  • Protein Name
    Rap1 GTPase-activating protein 1
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of RAP1GAP using anti-RAP1GAP antibody (A03561-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: human SH-SY5Y whole cell lysates, Lane 4: rat brain tissue lysates, Lane 5: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RAP1GAP antigen affinity purified polyclonal antibody (Catalog # A03561-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RAP1GAP at approximately 95 kDa. The expected band size for RAP1GAP is at 73 kDa.
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