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RAP1GAP Antibody

LS-C497248
LifeSpan BioSciences
ApplicationsWestern Blot
Product group Antibodies
ReactivityHuman
TargetRAP1GAP
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    RAP1GAP Antibody
  • Delivery Days Customer
    23
  • Application Supplier Note
    The predicted MW is 73kDa/75kDa/76kDa/80kDa, while the observed MW by Western blot was 90kDa.
  • Applications
    Western Blot
  • Applications Supplier
    WB (1:1000 - 1:2000) The predicted MW is 73kDa/75kDa/76kDa/80kDa, while the observed MW by Western blot was 90kDa.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID5909
  • Target name
    RAP1GAP
  • Target description
    RAP1 GTPase activating protein
  • Target synonyms
    RAP1GA1, RAP1GAP1, RAP1GAPII, RAPGAP, rap1 GTPase-activating protein 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Reactivity
    Human
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of RAP1GAP using anti-RAP1GAP antibody (A03561-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: human SH-SY5Y whole cell lysates, Lane 4: rat brain tissue lysates, Lane 5: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RAP1GAP antigen affinity purified polyclonal antibody (Catalog # A03561-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RAP1GAP at approximately 95 kDa. The expected band size for RAP1GAP is at 73 kDa.
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