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WB analysis of human testis lysate using GTX88813 RASSF8 antibody, Internal. Dilution : 0.3microg/ml Loading : 35microg protein in RIPA buffer
WB analysis of human testis lysate using GTX88813 RASSF8 antibody, Internal. Dilution : 0.3microg/ml Loading : 35microg protein in RIPA buffer
WB analysis of human testis lysate using GTX88813 RASSF8 antibody, Internal. Dilution : 0.3microg/ml Loading : 35microg protein in RIPA buffer

RASSF8 antibody, Internal

GTX88813
GeneTex
ApplicationsWestern Blot
DownloadDatasheet
Product group Antibodies
TargetRASSF8
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Overview

  • Supplier
    GeneTex
  • Product Name
    RASSF8 antibody, Internal
  • Delivery Days Customer
    7
  • Application Supplier Note
    WB: 0.3-1microg/ml. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.50 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID11228
  • Target name
    RASSF8
  • Target description
    Ras association domain family member 8
  • Target synonyms
    C12orf2; carcinoma-associated protein HOJ-1; HOJ1; Ras association (RalGDS/AF-6) domain family (N-terminal) member 8; ras association domain-containing protein 8
  • Host
    Goat
  • Isotype
    IgG
  • Protein IDQ8NHQ8
  • Protein Name
    Ras association domain-containing protein 8
  • Scientific Description
    This gene encodes a member of the Ras-assocation domain family (RASSF) of tumor suppressor proteins. This gene is essential for maintaining adherens junction function in epithelial cells and has a role in epithelial cell migration. It is a lung tumor suppressor gene candidate. A chromosomal translocation t(12;22)(p11.2;q13.3) leading to the fusion of this gene and the FBLN1 gene is found in a complex type of synpolydactyly. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2011]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of RASSF8 using anti-RASSF8 antibody (A11627-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human PC-3 whole cell lysates, Lane 3: human placenta tissue lysates, Lane 4: human U251 whole cell lysates, Lane 5: mouse testis tissue lysates, Lane 6: mouse lung tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RASSF8 antigen affinity purified polyclonal antibody (Catalog # A11627-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RASSF8 at approximately 48 kDa. The expected band size for RASSF8 is at 48 kDa.
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