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ProductsBack/Rat Anti-Mouse CD80-UNLB

Rat Anti-Mouse CD80-UNLB

1730-01
SouthernBiotech
ApplicationsFlow Cytometry, ImmunoPrecipitation, Neutralisation/Blocking
Product group Antibodies
TargetCd80
Price on request
0.5 mg
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Overview

  • Supplier
    SouthernBiotech
  • Product Name
    Rat Anti-Mouse CD80-UNLB
  • Delivery Days Customer
    7
  • Applications
    Flow Cytometry, ImmunoPrecipitation, Neutralisation/Blocking
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    1G10
  • Conjugate
    Unconjugated
  • Gene ID12519
  • Target name
    Cd80
  • Target description
    CD80 antigen
  • Target synonyms
    B71, Cd28l, Ly-53, Ly53, MIC17, TSA1, T-lymphocyte activation antigen CD80, B7 protein, activation B7-1 antigen
  • Host
    Rat
  • Isotype
    IgG2a
  • Protein IDQ00609
  • Protein Name
    T-lymphocyte activation antigen CD80
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Cd80 using anti-Cd80 antibody (A00196-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Daudi whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: mouse RAW264.7 whole cell lysates, Lane 4: mouse ANA-1 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Cd80 antigen affinity purified polyclonal antibody (Catalog # A00196-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Cd80 at approximately 70 kDa. The expected band size for Cd80 is at 33 kDa.
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