Rb antibody immunoprecipitates retinoblastoma 1 protein in IP experiments. IP samples: HeLa whole cell extract A. Control with 4 μg of preimmune Rabbit IgG B. Immunoprecipitation of retinoblastoma 1 protein by 4 μg Rb antibody (GTX100545) 7.5 % SDS-PAGE The immunoprecipitated retinoblastoma 1 protein was detected by Rb antibody (GTX100545) diluted at 1:500. [EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]
Rb antibody immunoprecipitates retinoblastoma 1 protein in IP experiments. IP samples: HeLa whole cell extract A. Control with 4 μg of preimmune Rabbit IgG B. Immunoprecipitation of retinoblastoma 1 protein by 4 μg Rb antibody (GTX100545) 7.5 % SDS-PAGE The immunoprecipitated retinoblastoma 1 protein was detected by Rb antibody (GTX100545) diluted at 1:500. [EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]
Rb antibody
GTX100545
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman
TargetRB1
Overview
- SupplierGeneTex
- Product NameRb antibody
- Delivery Days Customer9
- Application Supplier NoteICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. IP: 1:100-1:500. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration0.66 mg/ml
- ConjugateUnconjugated
- Gene ID5925
- Target nameRB1
- Target descriptionRB transcriptional corepressor 1
- Target synonymsOSRC, PPP1R130, RB, p105-Rb, p110-RB1, pRb, pp110, retinoblastoma-associated protein, prepro-retinoblastoma-associated protein, protein phosphatase 1, regulatory subunit 130, retinoblastoma 1, retinoblastoma suspectibility protein
- HostRabbit
- IsotypeIgG
- Protein IDP06400
- Protein NameRetinoblastoma-associated protein
- Scientific DescriptionThe protein encoded by this gene is a negative regulator of the cell cycle and was the first tumor suppressor gene found. The encoded protein also stabilizes constitutive heterochromatin to maintain the overall chromatin structure. The active, hypophosphorylated form of the protein binds transcription factor E2F1. Defects in this gene are a cause of childhood cancer retinoblastoma (RB), bladder cancer, and osteogenic sarcoma. [provided by RefSeq]
- ReactivityHuman
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC41116161
Datasheet
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![Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Rb antibody [HL2465] (GTX638814) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX638814/GTX638814_T-45096_20230714_WB_23071822_506.webp)
Product group Antibodies
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