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RBPMS Antibody, FITC conjugated

CSB-PA04569C0RB
Cusabio
ReactivityHuman
Product group Antibodies
TargetRBPMS
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Overview

  • Supplier
    Cusabio
  • Product Name
    RBPMS Antibody, FITC conjugated
  • Delivery Days Customer
    20
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    FITC
  • Gene ID11030
  • Target name
    RBPMS
  • Target description
    RNA binding protein, mRNA processing factor
  • Target synonyms
    HERMES, RNA-binding protein with multiple splicing, RNA binding protein with multiple splicing, heart and RRM expressed sequence
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ93062
  • Protein Name
    RNA-binding protein with multiple splicing
  • Scientific Description
    Acts as a coactivator of transcriptional activity. Required to increase TGFB1/Smad-mediated transactivation. Acts through SMAD2, SMAD3 and SMAD4 to increase transcriptional activity. Increases phosphorylation of SMAD2 and SMAD3 on their C-terminal SSXS motif, possibly through recruitment of TGFBR1. Promotes the nuclear accumulation of SMAD2, SMAD3 and SMAD4 proteins. Binds to poly(A) RNA.
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of RBPMS using anti-RBPMS antibody (A07130-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Raji whole cell lysates, Lane 2: rat liver tissue lysates, Lane 3: mouse NIH/3T3 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RBPMS antigen affinity purified polyclonal antibody (Catalog # A07130-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RBPMS at approximately 22KD. The expected band size for RBPMS is at 22KD.
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