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ProductsBack/RCHY1 / PIRH2 Antibody

RCHY1 / PIRH2 Antibody

LS-C831745
LifeSpan BioSciences
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetRCHY1
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    RCHY1 / PIRH2 Antibody
  • Delivery Days Customer
    23
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Applications Supplier
    ELISA (1:5000 - 1:10000), IHC (1:50 - 1:200), WB (1:500 - 1:2000)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID25898
  • Target name
    RCHY1
  • Target description
    ring finger and CHY zinc finger domain containing 1
  • Target synonyms
    ARNIP, CHIMP, PIRH2, PRO1996, RNF199, ZCHY, ZNF363, RING finger and CHY zinc finger domain-containing protein 1, CH-rich interacting match with PLAG1, E3 ubiquitin-protein ligase Pirh2, RING finger protein 199, RING-type E3 ubiquitin transferase RCHY1, androgen-receptor N-terminal-interacting protein, p53-induced protein with a RING-H2 domain, ring finger and CHY zinc finger domain containing 1, E3 ubiquitin protein ligase, zinc finger protein 363, zinc finger, CHY-type
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Pirh2/RCHY1 using anti-Pirh2/RCHY1 antibody (A04533-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human Daudi whole cell lysates, Lane 4: human 293T whole cell lysates, Lane 5: rat liver tissue lysates, Lane 6: rat PC-12 whole cell lysates, Lane 7: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Pirh2/RCHY1 antigen affinity purified polyclonal antibody (Catalog # A04533-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Pirh2/RCHY1 at approximately 21 kDa. The expected band size for Pirh2/RCHY1 is at 30 kDa.
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