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Western blot analysis of extracts of various cell lines using RECK Polyclonal Antibody at 1:1000 dilution.
Western blot analysis of extracts of various cell lines using RECK Polyclonal Antibody at 1:1000 dilution.
Western blot analysis of extracts of various cell lines using RECK Polyclonal Antibody at 1:1000 dilution.

RECK Polyclonal Antibody

E-AB-65539
Elabscience
Product group Antibodies
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Overview

  • Supplier
    Elabscience
  • Product Name
    RECK Polyclonal Antibody
  • Delivery Days Customer
    12
  • Certification
    Research Use Only
  • Scientific Description
    The protein encoded by this gene is a cysteine-rich, extracellular protein with protease inhibitor-like domains whose expression is suppressed strongly in many tumors and cells transformed by various kinds of oncogenes. In normal cells, this membrane-anchored glycoprotein may serve as a negative regulator for matrix metalloproteinase-9, a key enzyme involved in tumor invasion and metastasis. Several transcript variants encoding different isoforms have been found for this gene.
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of RECK using anti-RECK antibody (A06439-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MOLT-4 whole cell lysates, Lane 2: human U87 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RECK antigen affinity purified polyclonal antibody (Catalog # A06439-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RECK at approximately 110 kDa. The expected band size for RECK is at 110 kDa.
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Biomatik
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RECK Polyclonal Antibody, AbBy Fluor-350 ConjugatedBS-2901R-BF350
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
ReactivityBovine, Canine, Human, Mouse, Porcine, Rabbit, Rat, Sheep
TargetRECK
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IHC image of CSB-PA019536LA01HU diluted at 1:300 and staining in paraffin-embedded human liver tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
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RECK AntibodyCSB-PA019536LA01HU
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ReactivityHuman
TargetRECK
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Product group Antibodies
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RECK AntibodyCAB6718
ReactivityHuman
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Immunofluorescent staining of human cell line ASC TERT1 shows localization to plasma membrane.
Product group Antibodies
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TargetRECK
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Product group Antibodies
LifeSpan BioSciences
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WB analysis of various sample lysates using GTX66481 RECK antibody. The signal was developed with ECL plus-Enhanced. Dilution : 1:1000 Loading : 25μg per lane
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RECK antibodyGTX66481
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
ReactivityHuman, Mouse, Rat
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