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ProductsBack/Recombinant Anti-Podoplanin (MAP tag) (Clone PMab-1)

Recombinant Anti-Podoplanin (MAP tag) (Clone PMab-1)

TA386129
OriGene
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry, Neutralisation/Blocking
Product group Antibodies
TargetPdpn
200 ug
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Overview

  • Supplier
    OriGene
  • Product Name
    Recombinant Anti-Podoplanin (MAP tag) (Clone PMab-1)
  • Delivery Days Customer
    14
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry, Neutralisation/Blocking
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    PMab-1
  • Gene ID14726
  • Target name
    Pdpn
  • Target description
    podoplanin
  • Target synonyms
    E11, Gp38, OTS-8, RANDAM-2, T1-alpha, T1a, T1alpha, podoplanin, PA2.26 antigen, aggrus, glycoprotein 38, transmembrane glycoprotein E11
  • Host
    Rat
  • Isotype
    IgG2a
  • Protein IDQ62011
  • Protein Name
    Podoplanin
  • Scientific Description
    Recombinant Anti-Podoplanin (MAP tag) (Clone PMab-1)
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

MSDS

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Figure 1. Western blot analysis of Podoplanin/gp36/Pdpn using anti-Podoplanin/gp36/Pdpn antibody (A01124-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions. Lane 1: rat lung tissue lysates, Lane 2: mouse lung tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Podoplanin/gp36/Pdpn antigen affinity purified polyclonal antibody (Catalog # A01124-2) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Podoplanin/gp36/Pdpn at approximately 37KD. The expected band size for Podoplanin/gp36/Pdpn is at 37KD.
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