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Western blot analysis of anti-Ret Pab in SKBR3 cell lysate. Ret (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
Western blot analysis of anti-Ret Pab in SKBR3 cell lysate. Ret (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
Western blot analysis of anti-Ret Pab in SKBR3 cell lysate. Ret (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.

RET (N-term) Rabbit Polyclonal Antibody

AP14408PU-N
OriGene
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetRET
Price on request
400 ul
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Overview

  • Supplier
    OriGene
  • Product Name
    RET (N-term) Rabbit Polyclonal Antibody
  • Delivery Days Customer
    14
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Gene ID5979
  • Target name
    RET
  • Target description
    ret proto-oncogene
  • Target synonyms
    CDHF12, CDHR16, HSCR1, MEN2A, MEN2B, MTC1, PTC, RET-ELE1, proto-oncogene tyrosine-protein kinase receptor Ret, RET receptor tyrosine kinase, cadherin family member 12, cadherin-related family member 16, proto-oncogene c-Ret, rearranged during transfection, ret proto-oncogene (multiple endocrine neoplasia and medullary thyroid carcinoma 1, Hirschsprung disease)
  • Host
    Rabbit
  • Protein IDP07949
  • Protein Name
    Proto-oncogene tyrosine-protein kinase receptor Ret
  • Scientific Description
    RET (N-term) rabbit polyclonal antibody, Purified
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of RET using anti-RET antibody (A00293-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human THP-1 whole cell lysates Lane 2: human HL-60 whole cell lysates After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RET antigen affinity purified polyclonal antibody (Catalog # A00293-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RET at approximately 170KD. The expected band size for RET is at 124KD.
Product group Antibodies
Boster Bio
Anti-RET Antibody Picoband(r)A00293-1-CARRIER-FREE
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetRET
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Product group Antibodies
RayBiotech
Anti-RET Antibody101-11437
TargetRET
  • SizePrice
Product group Antibodies
Biosynth
Ret antibody70R-RG002
  • SizePrice
Product group Antibodies
Biomatik
Ret Recombinant AntibodyCAC12550
ApplicationsFlow Cytometry, ELISA
TargetRET
  • SizePrice
Product group Antibodies
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Ret Polyclonal AntibodyCAB0018
ReactivityHuman
TargetRET
  • SizePrice
Paraformaldehyde-fixed, paraffin embedded rat adrenal gland; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with RET Polyclonal Antibody, Unconjugated (bs-2793R) at 1:500 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.
Product group Antibodies
Bioss
RET Polyclonal AntibodyBS-2793R
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
ReactivityCanine, Human, Mouse, Rabbit, Rat
TargetRET
  • SizePrice
Western Blot analysis of various cells using Ret Polyclonal Antibody
Product group Antibodies
Cusabio
RET AntibodyCSB-PA003961
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ReactivityHuman, Mouse, Rat
TargetRET
  • SizePrice
Immunohistochemical staining of human adrenal gland shows strong membranous positivity in glandular cells.
Product group Antibodies
Atlas Antibodies
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ReactivityHuman
TargetRET
  • SizePrice
Product group Antibodies
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TargetRET
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Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with Ret antibody (GTX101059) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Product group Antibodies
GeneTex
Ret antibodyGTX101059
ApplicationsWestern Blot
ReactivityHuman
TargetRET
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