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ProductsBack/Rex1 antibody [5E11E7]

Rex1 antibody [5E11E7]

GTX60780
GeneTex
ApplicationsFlow Cytometry, Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman
TargetZFP42
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Overview

  • Supplier
    GeneTex
  • Product Name
    Rex1 antibody [5E11E7]
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1/500 - 1/2000. IHC-P: 1/200 - 1/1000. FACS: 1/200 - 1/400. ELISA: 1/10000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Flow Cytometry, Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    5E11E7
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID132625
  • Target name
    ZFP42
  • Target description
    ZFP42 zinc finger protein
  • Target synonyms
    REX-1, REX1, ZNF754, zfp-42, zinc finger protein 42 homolog, REX1 transcription factor, reduced expression protein 1, zinc finger protein 754
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDQ96MM3
  • Protein Name
    Zinc finger protein 42 homolog
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • Zeng YT, Liu XF, Yang WT, et al. REX1 promotes EMT-induced cell metastasis by activating the JAK2/STAT3-signaling pathway by targeting SOCS1 in cervical cancer. Oncogene. 2019,38(43):6940-6957. doi: 10.1038/s41388-019-0906-3
    Read this paper

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Figure 1. Western blot analysis of Rex1/ZFP42 using anti-Rex1/ZFP42 antibody (A08177-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human placenta tissue lysates, Lane 2: human Hela whole cell lysates, Lane 3: human Caco-2 whole cell lysates, Lane 4: human 22RV1 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Rex1/ZFP42 antigen affinity purified polyclonal antibody (Catalog # A08177-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Rex1/ZFP42 at approximately 45KD. The expected band size for Rex1/ZFP42 is at 45KD.
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