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Western Blot analysis of mouse liver mouse kidney cells using RFC1 Polyclonal Antibody
Western Blot analysis of mouse liver mouse kidney cells using RFC1 Polyclonal Antibody
Western Blot analysis of mouse liver mouse kidney cells using RFC1 Polyclonal Antibody

RFC1 Antibody

CSB-PA005450
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetRFC1
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Overview

  • Supplier
    Cusabio
  • Product Name
    RFC1 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID5981
  • Target name
    RFC1
  • Target description
    replication factor C subunit 1
  • Target synonyms
    A1, CANVAS, MHCBFB, PO-GA, RECC1, RFC, RFC140, replication factor C subunit 1, A1 140 kDa subunit, DNA-binding protein PO-GA, MHC binding factor, beta, RF-C 140 kDa subunit, activator 1 140 kDa subunit, activator 1 large subunit, activator 1 subunit 1, replication factor C (activator 1) 1, 145kDa, replication factor C 140 kDa subunit, replication factor C large subunit, replication factor C1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP35251
  • Protein Name
    Replication factor C subunit 1
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of RFC1 using anti-RFC1 antibody (PA1716). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: human Raji whole cell lysates, Lane 5: human K562 whole cell lysates, Lane 6: human CACO-2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RFC1 antigen affinity purified polyclonal antibody (Catalog # PA1716) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RFC1 at approximately 150 kDa. The expected band size for RFC1 is at 128 kDa.
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