More than 130 suppliers
ISO certified
In-house tech support
we Connect you
Bio-Connect
ProductsBack/RFC1 antibody
RFC1 antibody detects RFC1 protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: RFC1 protein stained by RFC1 antibody (GTX129291) diluted at 1:1000. Red: phalloidin, a cytoskeleton marker, diluted at 1:200. Blue: Hoechst 33342 staining. Scale bar = 10 μm.
RFC1 antibody detects RFC1 protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: RFC1 protein stained by RFC1 antibody (GTX129291) diluted at 1:1000. Red: phalloidin, a cytoskeleton marker, diluted at 1:200. Blue: Hoechst 33342 staining. Scale bar = 10 μm.
RFC1 antibody detects RFC1 protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: RFC1 protein stained by RFC1 antibody (GTX129291) diluted at 1:1000. Red: phalloidin, a cytoskeleton marker, diluted at 1:200. Blue: Hoechst 33342 staining. Scale bar = 10 μm.

RFC1 antibody

GTX129291
GeneTex
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry
Product group Antibodies
ReactivityHuman
TargetRFC1
Sign in to order and to see your custom pricing.
Large volume orders?
Order with a bulk request
More than 130 suppliers
ISO certified
In-house tech support

Overview

  • Supplier
    GeneTex
  • Product Name
    RFC1 antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IP: 1:100-1:500. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.13 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID5981
  • Target name
    RFC1
  • Target description
    replication factor C subunit 1
  • Target synonyms
    A1, CANVAS, MHCBFB, PO-GA, RECC1, RFC, RFC140, replication factor C subunit 1, A1 140 kDa subunit, DNA-binding protein PO-GA, MHC binding factor, beta, RF-C 140 kDa subunit, activator 1 140 kDa subunit, activator 1 large subunit, activator 1 subunit 1, replication factor C (activator 1) 1, 145kDa, replication factor C 140 kDa subunit, replication factor C large subunit, replication factor C1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP35251
  • Protein Name
    Replication factor C subunit 1
  • Scientific Description
    The protein encoded by this gene is the large subunit of replication factor C, which is a five subunit DNA polymerase accessory protein. Replication factor C is a DNA-dependent ATPase that is required for eukaryotic DNA replication and repair. The protein acts as an activator of DNA polymerases, binds to the 3 end of primers, and promotes coordinated synthesis of both strands. It also may have a role in telomere stability. [provided by RefSeq]
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • Cortese A, Simone R, Sullivan R, et al. Biallelic expansion of an intronic repeat in RFC1 is a common cause of late-onset ataxia. Nat Genet. 2019,51(4):649-658. doi: 10.1038/s41588-019-0372-4
    Read this paper

Datasheet

Download Datasheet

FAQ

Go to our frequently asked questions

Related products

Product group Antibodies
RayBiotech
Anti-RFC1 Antibody144-01625
ApplicationsImmunoFluorescence, Western Blot
ReactivityHuman
TargetRFC1
  • SizePrice
Paraformaldehyde-fixed, paraffin embedded Mouse lung; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with RFC1 Polyclonal Antibody, Unconjugated (bs-11304R) at 1:200 overnight at 4°C, DAB staining.
Product group Antibodies
Bioss
RFC1 Polyclonal AntibodyBS-11304R
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
ReactivityBovine, Canine, Chicken, Equine, Human, Mouse, Porcine, Rabbit, Rat
TargetRFC1
  • SizePrice
Western Blot analysis of mouse liver mouse kidney cells using RFC1 Polyclonal Antibody
Product group Antibodies
Cusabio
RFC1 AntibodyCSB-PA005450
ApplicationsWestern Blot, ELISA
ReactivityHuman, Mouse, Rat
TargetRFC1
  • SizePrice
Anti-RFC1 Antibody
Product group Antibodies
Antibodies.com
Anti-RFC1 AntibodyA101317
ApplicationsWestern Blot, ELISA
ReactivityHuman
  • SizePrice
ICC/IF analysis of MCF7 cells using GTX55779 RFC1 antibody. Blue : DAPI
Product group Antibodies
GeneTex
RFC1 antibodyGTX55779
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
ReactivityHuman
TargetRFC1
  • SizePrice
Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with RFC1 antibody [HL3242] (GTX640885) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Product group Antibodies
GeneTex
RFC1 antibody [HL3242]GTX640885
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
ReactivityHuman, Mouse, Rat
TargetRFC1
  • SizePrice
Product group Antibodies
Assay Genie
COX6C AntibodyCAB16250
ReactivityMouse
TargetRFC1
  • SizePrice
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.
Product group Antibodies
Atlas Antibodies
Anti-RFC1 AntibodyHPA046116
ApplicationsImmunoCytoChemistry
ReactivityHuman
TargetRFC1
  • SizePrice
Product group Antibodies
LifeSpan BioSciences
RFC1 / RFC AntibodyLS-C331592
ApplicationsImmunoFluorescence, Western Blot
ReactivityHuman
TargetRFC1
  • SizePrice
Immunoprecipitation of RFC1 protein from HeLa whole cell extracts using 5 μg of RFC1 antibody (GTX129292). Western blot analysis was performed using RFC1 antibody (GTX129292) diluted at 1:1500. EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.
Product group Antibodies
GeneTex
RFC1 antibodyGTX129292
ApplicationsImmunoPrecipitation, Western Blot
ReactivityHuman
TargetRFC1
  • SizePrice